Journal: Current protocols in chemical biology

Article Title: Universal non-antibody detection of protein phosphorylation using pIMAGO.

doi: 10.1002/9780470559277.ch140208

Figure Lengend Snippet: Figure 2 Procedural workflows of pIMAGO-based detection of phosphorylated proteins on (A) membrane or (B) 96-well plate. X = horseradish peroxidase or fluorophore conjugated to avidin. P denotes phosphoprotein.

Article Snippet: Materials Phosphorylated protein (e.g., β-casein) as a control Carbonate buffer (see recipe) Blocking buffer (see recipe) 1× TBST (see recipe) pIMAGO reagent (Tymora Analytical, cat. no. PMGO) pIMAGO buffer (see recipe) Avidin-peroxidase conjugate, for colorimetry-based detection (Sigma, cat. no. A3151) Avidin-fluorophore conjugate, for fluorescence-based detection (multiple suppliers with various fluorophores are available) Colorimetric TMB peroxidase substrate kit, for colorimetry-based detection (Bio-Rad, cat. no. 172-1066) 2% Oxalic acid prepared in water (protect from light) 96-well, clear High Bind polystyrene plate (Sigma, cat. no. CLS3590) Bind phosphoproteins in a 96-well plate and block 1.

Techniques: Membrane, Avidin-Biotin Assay

Journal: GeroScience

Article Title: Cerebral venous congestion promotes blood-brain barrier disruption and neuroinflammation, impairing cognitive function in mice

doi: 10.1007/s11357-019-00110-1

Figure Lengend Snippet: Effects on jugular vein ligation (JVL) on neurological parameters, grip strength, and motor skill learning. a JVL in mice resulted in a significant decline in the composite neuroscore. b Grip strength measured in mice with JVL tended to be weaker than the force values produced by sham-operated controls. c Motor skill learning on the accelerating rotarod. Daily changes in mean latencies to fall for mice with JVL and sham-operated controls are shown. Data are mean ± S.E.M. (n = 10–20 for each data point). *P < 0.05 vs. sham control

Article Snippet: Rotarod, motor skill learning Motor coordination was assessed in JVL and sham-operated control mice by using an automated four-lane rotarod (Columbus Instruments, Columbus, OH) as described (Tarantini et al. 2015 ).

Techniques: Ligation, Produced